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. 2022 Jun 2;12:878881. doi: 10.3389/fcimb.2022.878881

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Copyright © 2022 Wang, Wang, Liu, Wang, Wang, Xu, Huang and Gao

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Determination of the limit of detection (LOD) of the S. pneumoniae RPA-LFS assay. (A) The LOD of the established S. pneumoniae RPA-LFS assay was determined from 10 independent assays using the serially diluted genomic DNA of S. pneumonia, equivalent to 10⁴ to 10⁻¹ CFU. Images show the results of the RPA-LFS assays, and the amount of template is indicated at the top of the bar graph. (B) The group with 10 ng human genomic DNA added in addition to the P. gingivalis genomic DNA. (C) Probit regression analysis was performed on data collected from 10 replicates, using the SPSS software.