Table 1.
Primers and probes tested in this study.
| Name | Sequence (5’-3’) | Length (bp) | Amplicon size (bp) |
|---|---|---|---|
| lytA-1-F | ACAGAATGAAGCGGATTATCACTGGCGGAAAGA | 33 | 351 |
| lytA-1-R | GGATAAGGGTCAACGTGGTCTGAGTGGTTGTTTG | 34 | |
| lytA-2-F | CCGTACAGAATGAAGCGGATTATCACTGGCG | 31 | 355 |
| lytA-2-R | GGATAAGGGTCAACGTGGTCTGAGTGGTTGTTTG | 34 | |
| lytA-3-F | CAGAATGAAGCGGATTATCACTGGCGGAAAG | 31 | 369 |
| lytA-3-R | CCATTTAGCAAGATATGGATAAGGGTCAACG | 31 | |
| lytA-4-F | CATTGTTGGGAACGGTTGCATCATGCAGGTA | 31 | 281 |
| lytA-4-R | CGTGGTCTGAGTGGTTGTTTGGTTGGTTATTCG | 33 | |
| lytA-5-F | GCAGGTTTGCCGAAAACGCTTGATACAGGG | 30 | 154 |
| lytA-5-R | CATGCTTAAACTGCTCACGGCTAATGCCCCAT | 32 | |
| P1 | FITC-AATCTAGCAGATGAAGCAGGTTTGCCGAAA[THF] CGCTTGATACAGGGA-/C3-spacer/ | 46 | 125 |
| P2 | FITC-CAATCTAGCAGATGAAGCAGGTTTGCCGAA[THF] ACGCTTGATACAGGG-/C3-spacer/ | 46 | 113 |
| lytA-2-mR | Biotin-GGATAAGGGTCAACGTGGTCTGAGTGGTTGGTTG | 34 | / |
| lytA-4-mR | Biotin-CGTGGTCTGAGTGGTTGTTTGGTTGGTTAGTCG | 33 | / |
| mP1 | FITC-AGTCTAGCAGATGAAGCAGGTTTGCCGAAA[THF] CGCTAGATACAGGGA -/C3-spacer/ | 46 | / |
| mP2 | FITC-CAATGTAGCTGATGAAGCAGGTTTGCTGAA[THF] ACGCTTGATACAGGG-/C3-spacer/ | 46 | / |
| PCR-lytA-F | CAGATTTGCCTCAAGTCGGCGTGC | 24 | 691 |
| PCR-lytA-R | CCTGTAGCCATTTCGCCTGAGTTGTC | 26 |
Sequences modified with base substitutions. Modified bases are in red. F and R represent forward and reverse primers, respectively.