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. 2022 Jun 16;10:goac028. doi: 10.1093/gastro/goac028

Table 1.

Forward (F) and reverse (R) primer sequences for qRT–PCR

Gene Sequence
Mouse Gapdh F : 5′-AGGAGCGAGACCCCACTAACA-3′
R : 5′-AGGGGGGCTAAGCAGTTGGT-3′
Mouse Bmal1 F : 5′-GAACGGGGAAATACGGGTGA-3′
R : 5′-GCCTGTGACATTCTGCGAGGT-3′
Mouse Per1 F : 5′-CCCAGCTTTACCTGCAGAAG-3′
R : 5′-ATGGTCGAAAGGAAGCCTCT-3′
Mouse Per2 F : 5′-GGGAAACACCACGAGAATGAGAT-3′
R : 5′-GAGGGATTCTAGGCGCTTCATAG-3′
Mouse Cry1 F : 5′-TGAGGCAAGCAGACTGAATATTG-3′
R : 5′-CCTCTGTACCGGGAAAGCTG-3′
Mouse Cry2 R : 5′-CCTCTGTACCGGGAAAGCTG-3′
F : 5′-CTGGCGAGAAGGTAGAGTGG-3′
Mouse Clock F : 5′-GGCGTTGTTGATTGGACTAGG-3′
R : 5′-GAATGGAGTCTCCAACACCCA-3′
Mouse Nr1d1 F : 5′-CCGTGACCTTTCTCAGCATGA-3′
R : 5′-CACTGTCTGGTCCTTCACGTTG-3′
Human PER2 F : 5′-GCTGGCCATCCACAAAAAGA-3′
R : 5′-GCGAAACCGAATGGGAGAAT-3′
Human β-ACTIN F : 5′-TCACCCACACTGTGCCCATCTACGA-3′
R : 5′-CAGCGGAACCGCTCATTGCCAATGG-3′