Figure 3.

Systemic antitumor immune response is elicited after the combination of radiofrequency ablation with resiquimod. (A) Schematic diagram of the experiment to understand the different operations at different points in time. (B) Distant tumor growth curves of tumor-bearing mice treated with different approaches and the tumor weight (from two independent experiments) on the untreated side when the mice were sacrificed. (C, D) Tumors from the untreated side were dissected and made into single-cell suspensions for flow cytometry. The representative flow cytometry plots of tumor-infiltrating immune (CD45⁺) cells and natural killer (NK; CD3-NK1.1+) cells as well as the relative quantification of the ratio of CD45⁺ cells to live cells and the proportion of NK cells to CD45⁺ cells are shown. The results represent one of two independent experiments; n = 5 in each group. Statistical analysis was performed by two-tailed Student’s t-test. All error bars represent mean ± SEM. *p < 0.05; **p < 0.01; ns, not significant.