Figure 4.

The superimposed effects induced by combination therapy are natural killer (NK) cell dependent. (A) NK1.1 antibodies were injected intraperitoneally at 1 day prior to radiofrequency ablation (RFA) and once every 3 days until the mice were sacrificed at 3 days after injection tail vein blood was collected to verify the clearance efficiency. (B) Tumor growth curves of mice depleted for NK cells and treated with RFA + resiquimod. (C, D) Tumor weight and a representative picture of the tumor excised at sacrifice. One of the two independent experiments is shown; n = 5 in each group. Statistical analysis was performed by two-tailed Student’s t-test. All error bars represent mean ± SEM. *p < 0.05; **p < 0.01; ***p < 0.001; ns, not significant.