PL production during growth of C. luteola MFCL0. Cells were grown in PGA medium at 8°C (A), 17°C (B), and 28°C (C). PL activity was assayed as described in the text. Each datum point represents a mean based on at least three independent determinations. Symbols: ⧫, growth; ○, PL activity. The maximum standard deviation for any enzymatic determination was 5%, and the maximum standard deviation for growth was 4%. O.D. 580 nm, optical density at 580 nm.