FIG. 5.

Gel filtration of a concentrated culture supernatant from C. luteola MFCL0 on a Sephacryl S-300 HR column. The column was loaded with a concentrated supernatant from a culture grown at 14°C in PGA medium. Fractions (1 ml) were collected and assayed to determine their protein contents (○) and PL activities (⧫) as described in the text. Calibration of the column showed that fraction 30 corresponded to approximately 35 kDa.