Table 1. Quantum Yield and Brightness Measurements of WT iLOV and iLOVL470T/Q489Ka,b.
| WT | iLOVL470T/Q489K | |
|---|---|---|
| quantum yield (Φs) | 0.39 ± 0.06 (literature 0.3411) | 0.47 ± 0.07 (0.40 ± 0.07) |
| brightness | 4875 ± 750 (4250) | 5875 ± 781 (5000 ± 313) |
Numbers in parentheses are calculated by using the literature quantum yield of WT iLOV.
Linear regression plots used to calculate quantum yields are given in Figure S4.