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. 2022 Jun 15;13:68. doi: 10.1186/s40104-022-00716-0

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 6 — Effects of NMN supplementation on the transcriptome profiling of heat-stressed porcine oocytes. A Heatmap illustration showed the differentially expressed genes (DEGs) in control, heat-stressed and NMN-supplemented oocytes. B Volcano plot displayed the DEGs in heat-stressed oocytes compared to control oocytes. Red, upregulated; Blue, downregulated; Grey, not significant. C Volcano plot displayed the DEGs in NMN-supplemented oocytes compared to heat-stressed oocytes. Red, upregulated; Blue, downregulated; Grey, not significant. D RNA-seq results of selected genes in heat-stressed oocytes compared to control oocytes and NMN-supplemented oocytes compared to heat-stressed oocytes. E Validation of RNA-seq data by quantitative RT-PCR in control (blue), heat-stressed (red) and NMN-supplemented (green) oocytes. Data were presented as mean value (mean ± SEM) of at least three independent experiments. *P < 0.05, **P < 0.01. F KEGG pathway enrichment analysis of DEGs in heat-stressed oocytes compared to control oocytes. G KEGG pathway enrichment analysis of DEGs in NMN-supplemented oocytes compared to heat-stressed oocytes