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. 2022 Jun 15;29:42. doi: 10.1186/s12929-022-00824-z

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — Silencing of KRT17 downregulates cancer stemness in invasive OSCC. A KRT17 protein expressions in different OSCC cell lines were assessed by immunoblotting. B KRT17 mRNA expressions in the spheres formed from C9IV3 or HSC3 cells were analyzed by comparisons to their parental non-sphere C9IV3 or HSC3 cells, respectively, using qPCR. C Top, Protein expressions of KRT17 in C9IV3 and HSC3 cells transfected with Control-siRNAs (siCon) or three specific KRT17-siRNA plasmids (siKRT17-1, -2, -3) were assessed by immunoblotting. Middle, sphere-forming abilities of C9IV3 and HSC3 cells transfected with siCon or siKRT17 (siKRT17-3). Bottom, quantitative analysis of the number of spheres formed. D ALDH1, CD44 and CD133 expressions in spheres enriched from C9IV3 or HSC3 cells versus non-sphere cells were determined by qPCR. E ALDH1, CD44 and CD133 expressions in the C9IV3 or HSC3 spheres and non-sphere cells that had been transfected with siCon or siKRT17 were determined by qPCR. F Expressions of EMT markers Snail, Slug and Vimentin in the same spheres or non-sphere cells as in D, E. were determined by qPCR. Data are presented as mean ± SD (*p < 0.05, **p < 0.01 and ***p < 0.001)