FIGURE 9.

MERS-CoV perturbs colocalization between HSP70–1A and IKKε in infected cells. FLAG-IKKε and myc-HSP70–1A expression constructs were transfected into Huh-7 cells. Cells were either mock infected or infected with WT or Δ8b virus at 24 h posttransfection with an MOI of 0.01. Cells were fixed and labeled with primary Abs against epitope tag and N protein of MERS-CoV at 24 hpi. Cells were incubated with secondary Abs conjugated with fluorophores after primary Ab incubation and subsequently visualized by confocal microscope. Nuclei were costained with DAPI with secondary Abs. DAPI is in blue. IKKε is in green. HSP70–1A is in red. MERS-N is in pink. Scale bar, 20 μm.