FIG 1.
rECOL MAbs bind to the conserved outer membrane protein (OmpA). (A) Enzyme-linked immunosorbent assays (ELISA) of recombinant monoclonal antibody binding to E. coli lysate [BL21(DE3), K-12, and UTI89] or recombinant protein (rOmpACTerm). Points represent arithmetic mean, error bars represent standard deviation, curves indicate four-parameter variable slope logistic analysis, and data are representative of three independent experiments performed in quadruplicate. (B) Clustal Omega alignment of OmpA from the strains tested by ELISA, namely, an engineered E. coli (BL21), a laboratory or commensal strain (K-12 MG1655), and a clinical uropathogenic strain (UTI89), shows 99% identity (black, conserved; white, variable). Orange indicates the N-terminal porin domain with secondary structure above mapped from PDB 1BXW using ESPript 3; green indicates the C-terminal periplasmic domain with secondary structure below mapped from PDB 2MQE.