FIG 2.

Induction of M Cell formation in Enteroids and HIOs Enhances STm Invasion. Addition of RANKL (100 ng/mL) enriches M cells, as determined by GP2 staining in Enteroids (A) and HIOs (B). RANKL significantly increases GP2 and Spi-B mRNA (C, D) but not RANK receptor (E). WT STm (0.01 OD₆₀₀) was applied for 2 h to monolayers treated with and without RANKL. Results show RANKL treated monolayers have significantly increased STm invasion in a gentamicin invasion assay (F) *, P < 0.05, one-way ANOVA with Tukey posttest. Confocal images shown are representative images from (n = 3) biological replicates.