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. 2022 May 17;90(6):e00041-22. doi: 10.1128/iai.00041-22

FIG 5.

FIG 5

Expression of pIgR and FCGRT in Enteroids and Organoids for Sal4 transcytosis. Effect of IL-1 on pIgR (A) and FCGRT (B) expression in Enteroids and HIOs. Treatments shown are no IL-1 and 10 ng/mL IL-1. Confocal microscopy shows IL-1 significantly increases pIgR staining relative to control in both Enteroids and HIOs, but IL-1 does not have an effect on FCGRT expression. The effect of IL-1 was also determined by measuring relative mRNA levels of pIgR (C) and FCGRT (D). Results show IL-1 significantly increases pIgR relative to control in both Enteroids and HIOs, but IL-1 does not have an effect on FCGRT expression. Transcytosis of IgA and IgG was quantified with an ELISA using STm LPS (E). MAbs (10 µg/mL Sal4 dIgA, Sal4 mIgA and Sal4 IgG) were added to monolayers basolaterally for 16 h. Apical medium was collected for ELISA. Results show dIgA and IgG was transported through the membrane, but the monomers were not. The bar graphs depict combined data from biological replicates (n = 3) P < 0.05, one-way ANOVA with Tukey post test.