FIG. 3.

Comparative analyses of polyclonal anti-GroEL immunoprecipitates. (a) 10% SDS-PAGE of precipitated proteins; (b and c) Western blot analyses of Ki-4(scFv)-ETA′ and GroEL, respectively. Results are shown for standard growth conditions (LB medium with 150 mM NaCl) (lanes 1 through 3) and osmotic-stress growth conditions in the presence of 10 mM glycine betaine (lanes 4 through 6). Lanes 1 and 4, before stress and IPTG induction; lane 2, 1/2 h before IPTG induction; lane 5, 1/2 h after supplementation with NaCl, sorbitol, and glycine betaine; lanes 3 and 6, 2 h after IPTG induction. Arrowhead indicates the position of Ki-4(scFv)-ETA′.