TABLE 1.
Plasmids, bacteria, and eukaryotic cells used in this study
| Designation | Function |
|---|---|
| Plasmids | |
| pcDNA3 | Eukaryotic expression of recombinant soluble CD30 receptor |
| pCANTAB6 | Phagemid vector used for the synthesis of filamentous phages displaying recombinant scFv's on their surfaces |
| pBM1.1 | Bacterial vector for periplasmic expression of recombinant immunotoxins |
| Bacteria | |
| XL1-Blue | Cloning and sequencing |
| TG1 | Synthesis of scFv-displaying phages |
| HB2151 | Expression of soluble scFv |
| BL21(DE3) | Periplasmic expression of recombinant immunotoxins |
| Cells | |
| COS-1 | Expression of soluble CD30 receptor |
| Ki-4 | Anti-CD30 MAb producing hybridoma |
| RFT5 | Anti-CD25 MAb producing hybridoma |
| HD-MyZ | CD25− CD30− Hodgkin-derived cell line |
| L540Cy | CD25+ CD30+ Hodgkin-derived cell line |