| Reagent, Equipment | Vendor | Catalog Number |
|---|---|---|
| RNaseZAP | Sigma-Aldrich | R2020 |
| 1.5ml Rnase-free tubes (3 per probe set) | ||
| HCR ssDNA Probes | Molecular Instruments | |
| Fluorescent HCR amplifier hairpins | Molecular Instruments, Alexa Fluor® | HCR Amplifier |
| Nuclease-free H2O | Ambion | AM9932 |
| Negative 20°C freezer for storage | ||
| An excess of 1–10 μl pipette tips | ||
| 8-channel 1–10 μl pipette | ||
| Repeat piettor with 200 μl capacity | ||
| PCR tubes, 8-tube strip with attached caps | ||
| 50 ml conical tubes (2 per probe set) | ||
| 1000 μl pipette | ||
| 96-well plastic tube rack (treat with bleach, then RNase decontamination solution) |
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