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. 2022 Jun 2;9:885134. doi: 10.3389/fvets.2022.885134

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Copyright © 2022 Huma, Sharma, Kour and Lee.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Amplified PCR products of E. coli gene isolated from mastitis milk samples. (a) PCR product of E. coli gene at 232 bp. Lane M: 100 bp ladder; Lane 1: negative control; Lanes 2: positive control; Lane 3, 4, 5, 7, 8, and 9: are E. coli gene positive; Lane 6: is E. coli gene negative; (b) PCR product of stx1gene at 180 bp. M: 100 bp ladder; Lane 1: positive control; Lanes 2, 3, 4, 6, 7, and 8: are stx1 gene positive; Lane 5: is stx1gene negative; Lane 9: negative control; (c) PCR product of stx2 gene at 255 bp. M: 100 bp ladder; Lane 8: positive control; Lanes 2, 3: are stx2 gene positive; Lane 1, 4, 6, 7, and 5: are stx2 gene negative; Lane 9: negative control; (d) PCR product of hly gene at 180 bp. M: 100 bp ladder; Lane 1: positive control; Lanes 2, 5, 7, 8: are hly gene positive; Lane 3, 4, 6: are hly gene negative; Lane 9: negative control.