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. Author manuscript; available in PMC: 2022 Jun 16.
Published in final edited form as: Cell Rep. 2021 Sep 21;36(12):109742. doi: 10.1016/j.celrep.2021.109742

Figure 6. LrpprcBKO mice exhibit improved systemic metabolism due to ATF4 activation in brown adipocytes.

Figure 6.

(A) Body weight of male CON and LrpprcBKO mice under 12-week HFD at RT and 30°C. Sample size: CON/RT (n=12), LrpprcBKO/RT (n=11), CON/30°C (n=7) and LrpprcBKO/30°C (n=15). (B) Representative images of dissected iWAT, eWAT and BAT from male CON and LrpprcBKO mice after 12-week HFD. Serum glucose levels during ITT in male CON and LrpprcBKO mice after 4-week and 12-week HFD at RT (C) and 30°C (D). Sample size: CON/4w-HFD/RT (n=8), LrpprcBKO/4w-HFD/RT (n=7), CON/12w-HFD/RT (n=5), LrpprcBKO/12w-HFD/RT (n=5), CON/4w-HFD/30°C (n=6), LrpprcBKO/4w-HFD/30°C (n=9), CON/12w-HFD/30°C (n=7) and LrpprcBKO/12w-HFD/30°C (n=15). (E) Area under the curve (AUC) values of glucose levels in ITTs showed. (F) Serum insulin levels in male CON and LrpprcBKO mice after 12-week HFD. Sample size: CON/RT (n=5), LrpprcBKO/RT (n=4), CON/30°C (n=4), LrpprcBKO/30°C (n=6). (G) Serum triglyceride contents of male CON and LrpprcBKO mice after HFD at RT. Sample size: Sample size: CON/NC/RT (n=6), LrpprcBKO/NC/RT (n=10), CON/4w-HFD/RT (n=8), LrpprcBKO/4w-HFD/RT (n=10), CON/12w-HFD/RT (n=5) and LrpprcBKO/12w-HFD/RT (n=5). (H) Serum triglyceride contents of male CON and LrpprcBKO mice after 12-week HFD at 30°C. Sample size: CON/12w-HFD/30°C (n=6) and LrpprcBKO/12w-HFD/30°C (n=13). (I) Representative H&E staining of liver from male CON and LrpprcBKO mice after 12-week HFD. Scale bar: 25 μm. (J) Liver triglyceride contents of male CON and LrpprcBKO mice after HFD at RT. Sample size: CON/NC/RT (n=6), LrpprcBKO/NC/RT (n=7), CON/4w-HFD/RT (n=7), LrpprcBKO/4w-HFD/RT (n=6), CON/12w-HFD/RT (n=5) and LrpprcBKO/12w-HFD/RT (n=5). (K) Liver triglyceride contents of male CON and LrpprcBKO mice after 12-week HFD at 30°C. Sample size: CON/12w-HFD/30°C (n=7) and LrpprcBKO/12w-HFD/30°C (n=14). (L) Body weight of male CON, LrpprcBKO and Lrpprc;Atf4BKO mice under 12-week HFD at RT. Sample size: CON/RT (n=13), LrpprcBKO/RT (n=6) and Lrpprc;Atf4BKO/RT (n=8). (M) Serum glucose levels during ITT in male CON, LrpprcBKO and Lrpprc;Atf4BKO mice after 12-week HFD at RT. (N) Area under the curve (AUC) values of glucose levels in ITTs showed. (O) Serum insulin levels of male CON, LrpprcBKO and Lrpprc;Atf4BKO mice after HFD at RT. (P) Representative H&E staining of liver from male CON, LrpprcBKO and Lrpprc;Atf4BKO mice after 12-week HFD at RT. Scale bar: 25 μm. Liver triglyceride contents (Q) and serum triglyceride contents (R) of male CON, LrpprcBKO and Lrpprc;Atf4BKO mice after HFD at RT. Sample size: CON (n=11), LrpprcBKO (n=5), and Lrpprc;Atf4BKO (n=6). (S) Body weight of male CON, LrpprcBKO and Lrpprc;Atf4BKO mice under 12-week HFD at 30°C. Sample size: CON/30°C (n=11), LrpprcBKO/30°C (n=7), and Lrpprc;Atf4BKO/30°C (n=6). (T) Serum glucose levels during ITT in male CON, LrpprcBKO and Lrpprc;Atf4BKO mice after 12-week HFD at 30°C. (U) Area under the curve (AUC) values of glucose levels in ITTs showed. (V) Serum insulin levels of male CON, LrpprcBKO and Lrpprc;Atf4BKO mice after HFD at 30°C. (W) Representative H&E staining of liver from male CON, LrpprcBKO and Lrpprc;Atf4BKO mice after 12-week HFD at 30°C. Scale bar: 25 μm. Liver triglyceride contents (X) and serum triglyceride contents (Y) of male CON, LrpprcBKO and Lrpprc;Atf4BKO mice after HFD at 30°C. Sample size: CON (n=9), LrpprcBKO (n=6), and Lrpprc;Atf4BKO (n=5). Data were presented as average ± SEM. Unpaired t-test. *: p<0.05 and **: p<0.01.