KEY RESOURCES TABLE.
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Rabbit anti-Lrpprc | Santa Cruz Biotechnology |
Cat#SC-66844 |
| Rabbit anti-phospho-PKA substrates | Cell Signaling | Cat#9624 |
| Rabbit anti-p-eIF2α | Cell Signaling | Cat#3398 |
| Rabbit anti-eIF2α | Cell Signaling | Cat#5324 |
| Rabbit anti-p-S6 | Cell Signaling | Cat#5364 |
| Rabbit anti-S6 | Cell Signaling | Cat#2217 |
| Rabbit anti-p-4Ebp1 | Cell Signaling | Cat#2855 |
| Rabbit anti-4Ebp1 | Cell Signaling | Cat#9452 |
| Rabbit anti-Atf4 | ABclonal | Cat#A0201 |
| Mouse anti-total OXPHOS rodent | Abcam | Cat#ab110413 |
| Rabbit anti-Hsp60 | Bethyl | Cat#A302-846A |
| Rabbit anti-mt-Co2 | Proteintech | Cat#55070-1-AP |
| Rabbit anti-Cox4 | Cell Signaling | Cat#4850 |
| Rabbit anti-Cox5b | Bethyl | Cat#A-305-523A-T |
| Rabbit anti-Cox6b | Abgent | Cat#AP20624a |
| Rabbit anti-Slc3a2 | Proteintech | Cat#15193-1-AP |
| Rabbit anti-Puromycin | Kerafast | Cat#EQ0001 |
| Mouse anti-Ubiquitin | Santa Cruz Biotechnology |
Cat#SC-8017 |
| Rabbit anti-Ucp1 | Sigma | Cat#U6382 |
| Mouse anti-Flag | Sigma | Cat#F1804 |
| Rabbit anti-Hsp90 | Santa Cruz Biotechnology |
Cat#SC-7949 |
| Rabbit anti-Lc3b | Cell Signaling | Cat#2775 |
| Rabbit anti-Hsl | Cell Signaling | Cat#4107 |
| Rabbit anti-Atgl | Cell Signaling | Cat#2138 |
| Rabbit anti-Actin | Cell Signaling | Cat#4967 |
| Bacterial and Virus Strains | ||
| GFP adenovirus | Vector biolabs | Cat#1060 |
| Cre adenovirus | Vector biolabs | Cat#1700 |
| Biological Samples | ||
| N/A | ||
| Chemicals, Peptides, and Recombinant Proteins | ||
| B-NADH | Sigma | Cat#N7410 |
| DCIP | Sigma | Cat#D1878 |
| DTNB | Sigma | Cat#D8130 |
| Acetyl Coenzyme A | Sigma | Cat#A2056 |
| Cytochrome C | Sigma | Cat#C3131 |
| Decylubiquinone | Sigma | Cat#D7911 |
| Potassium Borohydride | Sigma | Cat#P4129 |
| Potassium Cyanide | Sigma | Cat#20781 |
| Potassium Ferricyanide | Sigma | Cat#244023 |
| Sodium Hydrosulfite | Sigma | Cat#157953 |
| MG132 | Millipore | Cat#474791 |
| diphtheria toxin | Biological Laboratories Inc |
Cat#150 |
| Puromycin | ThermoFisher | Cat#A1113803 |
| Rapamycin | TCI America | Cat#TCR0097 |
| Bortezomib | Selleck | Cat#S1013 |
| Insulin solution human | Sigma | Cat#I9278-5ML |
| 2-amino-2-norbornane-carboxylic acid (BCH) |
Sigma | Cat#A7902 |
| α-methyl-dl-tryptophan (αMT) | Sigma | Cat#M8377 |
| Orlistat | Selleck | Cat#S1729 |
| Sotagliflozin | Selleck | Cat#M8377 |
| propranolol | Sigma | Cat#P0884 |
| CL316243 disodium salt | Tocris bioscience | Cat#1499 |
| Bio-Rad Protein Assay Solution | BioRad | Cat#500-0006 |
| TRIsure | Bioline | Cat#BIO-38033 |
| Collagenase | Sigma | Cat#C6885 |
| Dispase II | Roche | Cat##14549000 |
| Forskolin | Sigma | Cat#F6886 |
| 3,3′,5-Triiodo-L-thyronine (T3) | Sigma | Cat#T2877 |
| 3-Isobutyl-1-methylxanthine (IBMX) | Sigma | Cat#I5879 |
| Dexamethasone | Sigma | Cat#D8893 |
| Indomethacin | Sigma | Cat#I7378 |
| Rosiglitazone | Sigma | Cat#R2408 |
| Poly-L-Lysine | Sigma | Cat#P2636 |
| Critical Commercial Assays | ||
| Infinity Triglycerides Reagent | Thermo Scientific | Cat#TR22421 |
| Free Glycerol Reagent | Sigma | Cat#F6428 |
| QIAamp DNA Mini Kit | Qiagen | Cat#51304 |
| ISOLATE II RNA Mini Kit | Bioline | Cat#BIO-52073 |
| iScript cDNA Synthesis Kit | BioRad | Cat#170-8891 |
| Mouse insulin ultrasensitive ELISA kit | Alpco | Cat#80-INSMSV-E01 |
| Mouse leptin ELISA kit | Crystal Chem Inc | Cat#90030 |
| Mouse/Rat FGF-21 Quantikine ELISA Kit | R&D | Cat#MF2100 |
| L-Lactic Acid (L-Lactate) Assay Kit | Megazyme | Cat#K-LATE |
| SILAC Protein Quantitation Kit | Thermo Scientific | Cat##A33969 |
| Deposited Data | ||
| BAT mitochondrial mass spectrometry data | ProteomeXchange Consortium |
PXD008798 |
| BAT RNA-seq data | NCBI GEO | GSE117985 |
| Experimental Models: Cell Lines | ||
| N/A | ||
| Experimental Models: Organisms/Strains | ||
| ROSA-LSL-FlaghATF4 | JAX | Cat#029394 |
| ROSA-LSL-iDTR | JAX | Cat#007900 |
| Ucp1 KO | JAX | Cat#003124 |
| Lrpprcf/f | (Ruzzenente et al., 2012) | |
| Ucp1-Cre | JAX | Cat#024670 |
| Atf4f/f | (Ebert et al., 2012) | N/A |
| TfamBKO | (Masand et al., 2018) | N/A |
| Lkb1BKO | (Masand et al., 2018) | N/A |
| GnasBKO | (Paulo et al., 2018b) | N/A |
| betaless | (Bachman et al., 2002) | N/A |
| Oligonucleotides | ||
| Full sequences in Supplementary Table 4 | Elim Biopharm | N/A |
| Recombinant DNA | ||
| N/A | ||
| Software and Algorithms | ||
| ImageJ | NIH | |
| MaxQuant data analysis | (Cox et al., 2014) | |
| Adiposoft | (Galarraga et al., 2012) | |
| AMIDE | amide.sourceforge.org | |
| Cytoscape | cytoscape.org | |
| NetworkAnalyst | www.networkanalyst.ca/ | |
| FLIR QuickReport | www.flir.com | |
| Other | ||
| Low-protein diet (LP) | Research Diets | Supplementary Table 3 |
| Isogenic control protein diet (CP) | Research Diets | Supplementary Table 3 |