Figure 4.

Creeping cells aggregate over time. Mating and nonmating samples of a+ α cells or haploid a cells were observed under the microscope. Images were taken from 2 to 7 h after cells were mixed. Representative images at ×100 and ×400 magnification are shown. Extensive cell aggregation is observed for mating cells from 4 h following mixing, while no appreciable aggregation is observed for nonmating cells over the same time period. See Fig. S4 (Supporting Information) for the full time course.