Figure 3.

Immunolocalization of anti-Müllerian hormone type II receptor (AMH-R2; using the custom-made antibody) with α-tubulin or A-kinase anchor protein 4 (AKAP4) in human ejaculated spermatozoa. In human ejaculated spermatozoa, AMH-R2 (red; anti-AMH-R2 antibody) is expressed in the head, middle piece, and tail. (A) α-Tubulin (green) is expressed in the middle piece, and tail. (B) AKAP4 (green) is expressed only in the tail. (C) Absence of staining without primary antibodies. Only secondary antibodies and diaminopyrolylindole 4,6-diamino, 2-pyrolylindole nuclear counterstaining (blue) were used. Scale bars = 5 μ>m.