Figure 1.

Flow cytometry gating strategy for quantification of human type II NKT cells. (A) Human T2NKT cells were defined as CD3⁺ CD56⁺ CD161⁺ TCRγδ^- TCRVα7.2^- TCRVα24^- lymphocytes. Gating of human PBMC resulted in a very low frequency of circulating T2NKT cells (0.3% of total CD3⁺ T cells). (B) Gating of human intrahepatic lymphocytes (IHL) revealed a higher frequency of T2NKT cells in non-steatotic liver (6.5% of total CD3⁺ T cells). Intrahepatic T2NKT cells could be subdivided into CD4⁺ and CD8⁺ cells. (C) Frequency of human T2NKT cells with respect to CD3⁺ T cells from 3 PBMC and 3 liver samples (steatosis 0%, n = 2; steatosis 15%, n = 1).