Table 1.

Characteristics of frequently used molecular approaches for typing Mycoplasma pneumoniae and Mycoplasma genitalium (results of studies with >25 patients are included; if not presented in the study, HGDI’s are calculated according to the data).

Species	Typing method	Number of known types	HGDI	References
M. pneumoniae	p1	15	0.42–0.68	Brown et al., 2015; Dumke et al., 2015; Touati et al., 2015; Kenri et al., 2020; Xiao et al., 2020; Dumke and Rodriguez, 2021; Meyer Sauteur et al., 2021
	MLVA	24	0.58–0.68	Benitez et al., 2012; Brown et al., 2015; Dumke et al., 2015; Touati et al., 2015; Kogoj et al., 2018; Zhao et al., 2019; Kenri et al., 2020; Xiao et al., 2020; Dumke and Rodriguez, 2021; Meyer Sauteur et al., 2021
	MLST	46	0.66–0.78	http://pubmlst.org/mpneumoniae; Brown et al., 2015; Kenri et al., 2020; Dumke and Rodriguez, 2021; Meyer Sauteur et al., 2021
	SNP	15	0.80–0.84	Touati et al., 2015; Kenri et al., 2020; Dumke and Rodriguez, 2021
	p1 + MLVA; p1 + MLST; p1 + SNP; MLVA + SNP; and MLST + SNP	n.d.	0.60–0.88	Brown et al., 2015; Dumke et al., 2015; Touati et al., 2015; Kenri et al., 2020; Xiao et al., 2020; Dumke and Rodriguez, 2021; Meyer Sauteur et al., 2021
	p1 + MLVA + MLST; p1 + MLVA + SNP	n.d.	0.75–0.91	Brown et al., 2015; Touati et al., 2015; Kenri et al., 2020; Dumke and Rodriguez, 2021; Meyer Sauteur et al., 2021
	p1 + MLVA + MLST + SNP	n.d.	0.88–0.90	Kenri et al., 2020; Dumke and Rodriguez, 2021
M. genitalium	mgpB	246	0.82–0.99	Hjorth et al., 2006; Ma et al., 2008; Cazanave et al., 2012; Olsen et al., 2012; Dumke et al., 2020; Fernandez-Huerta et al., 2020b; Plummer et al., 2020; Sweeney et al., 2020; Chua et al., 2021; Dumke and Spornraft-Ragaller, 2021; Guiraud et al., 2021
	VNTR MG_309	12	0.84–0.95	Ma et al., 2008; Cazanave et al., 2012; Dumke and Spornraft-Ragaller, 2021; Guiraud et al., 2021; Laumen et al., 2021
	mgpB + VNTR MG_309	n.d.	0.95–0.99	Ma et al., 2008; Cazanave et al., 2012; Dumke and Spornraft-Ragaller, 2021; Guiraud et al., 2021

p1, sequence differences in p1 gene (MPN141); MLVA, multilocus variable number of tandem-repeat analysis; MLST, multilocus sequence typing; SNP, determination of single polynucleotide polymorphisms; mgpB, sequence differences in mgpB gene (MG_191); VNTR MG_309, variable number of tandem repeat in gene MG_309; HGDI, discriminatory index (Hunter and Gaston, 1988); and n.d., not determined.