Figure 5.

Comparison of the relative expression levels of target genes in two RILs. (A) qRT-PCR analyses of target genes of ahy-miR160, ahy-miR162, and ahy-miR396 expression levels in two peanut RILs upon low-temperature (6°C) treatment at the indicated time points, respectively. (B) qRT-PCR analyses of target genes of ahy-miR482, ahy-miR1511, and ahy-miR2118 expression levels in two peanut RILs upon low-temperature (6°C) treatment at the indicated time points, respectively. Data are shown as means ± SD (n = 3). Student’s t-test was used to determine the significance of differences between 0 HCT and the indicated time points. Asterisks indicate significant differences (^*p < 0.05 and ^**p < 0.01). Similar results were obtained from three biological replicates.