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XIST promotes iron overload-induced INS-1 cells injury by regulating miR-130a-3p/ALK2 axis. (a) The expression of ALK2 protein was detected by Western blot. (b) Cell proliferation was measured by MTT assay. (c) Representative results of TUNEL staining for every group. Scale bar = 100 μm. (d) Knockdown of XIST downregulated LIP. (e) GSIS assay was used to measure the secretion of insulin. (f) The protein expression was measured by Western blot. ∗P < 0.05, ∗∗P < 0.01.
