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. 2022 Jun 6;24(6):872–884. doi: 10.1038/s41556-022-00925-9

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 4 — (a) Representative confocal microscopy images of HK2 (green) and Tom20 (red) in FACS sorted CD34+ and CD34- cord blood cells. Scale bar = 10µm. The white (HK2), red (Tom20) and blue (DAPI) curve in the scan profiles represents the fluorescence intensity of HK2, Tom20 and DAPI along the plane. Representative images from 3 biologic samples. (b) Gating strategy for FACs sorted CD34+ and CD34- cord blood cells. (c) Vav promoter flanked cDNA construct of HK2 fused in frame with a nuclear localizing signal. (d) Body weight (n = 42 wild-type control mice, n = 22 Vav-NLS-HK2 mice) and body length (e) (n = 48 wild-type control mice, n = 31 Vav-NLS-HK2 mice) of Vav NLS-HK2 transgenic mice and control littermates. (f) Complete blood count analysis of Vav NLS-HK2 transgenic mice and control littermates (n = 21 wild-type control mice, n = 13 Vav-NLS-HK2 mice). Statistical analyses for experiments (d-e) was performed using an unpaired Student’s t-test. Data represent mean +/- s.e.m.

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