Extended Data Fig. 7. Human blastoids.
a–b. Boxplots with the immunofluorescence quantification at 36 h (a) and 60 h (b) blastoids with or without PRC2i. Blastoids were stained for NANOG, GATA3 and FOXA2. The boxplots show the interquartile range (box limits) and median (centre line) of the total number of positive cells per blastoid (left panel). The right panel indicates the total number of cells per blastoid. N = 21 blastoids for 36 h -PRC2i blastoids; n = 23 blastoids for 36 h + PRC2i; n = 27 blastoids for 60 h -PRC2i; and n = 17 blastoids for 60 h + PRC2i, quantified from n = 1 experiment. A two-sided Wilcoxon rank-sum test with Bonferroni correction was used for significance testing. 36 h: ****P = 1 ×10-6; 60 h: ****P = 3.9 ×10-5, **P = 1.6 ×10-3. c. Close-up for average number of FOXA2 + cells as found in (b). N = 27 blastoids for -PRC2i, and n = 17 blastoids for +PRC2i, quantified from n = 1 experiment. PrEnd = Primitive Endoderm. d. Representative immunofluorescence images for Fig. 7c and Extended Data Fig. 7a-b. NANOG is shown in green, GATA3 in magenta and FOXA2 in yellow. Scale bar: 200 mm. The experiment was performed once. e. Immunofluorescence analysis of human blastoids with and without PRC2i. Cells were stained for AQP3 (white) and NANOG (green) after 24 h. Representative image from 1 experiment. Scale bar: 200 mm. f. Quantification of cavitated human blastoids after 36 and 60 h with and without PRC2i. N = 3 biologically independent samples. g. Quantification of immunofluorescence in naïve human pluripotent stem cells cultured in PXGL treated with PRC2i EPZ-6438 or UNC1999 for 7 days. Cells were stained for GATA3 and GATA4. The experiment was performed once. Underlying source data is provided in Source Data Extended Data Fig. 7.