FIG. 2.

Southern blot analysis of six PCR amplified genes, demonstrating the homokaryotic nature of RP-78. For each gene, two identical gels were run, with BKM-F-1767 PCR products in the left lanes and RP-78 products in the right. Each gel was then blotted to Nytran and probed with an allele-specific oligonucleotide. The blots show both alleles present in the dikaryotic strain BKM-F-1767 but only a single allele present in RP-78. PCR product sizes were as follows: lipE, 1,535 nucleotides (nt); lipJ, 675 nt; lipF, 1,480 nt; lipD, 440 nt; glx, 680 nt; and mnp2, 1,509 nt.