Fig. 6. tSV are enriched in proteins related to ubiquitination and RNA-binding proteins.

a–b Venn-diagrams showing the overlap of enriched proteins identified in the tSV and EVs of TH (a) and CTL (b). c–f Pie charts showing the enriched protein classes identified with PANTHER⁴⁸ in the tSV of TH (c) and CTL (d), and in their syngeneic EV counterparts (e–f). g–j GSEA using PANTHER⁸⁶ revealed a greater enrichment of molecular function categories related to nucleic acid-binding and ubiquitination in the proteomics of tSV derived from TH (g) and CTL (h) as compared with syngeneic EVs of TH (i) and CTLs (j). Data from n = 4 biologically independent samples (donors, a–j) across four independent experiments. Statistical analysis for functional and biological pathway enrichment analyses (c–f) using PANTHER was performed by the Fisher’s exact test and the Benjamini–Hochberg False Discovery Rate (FDR) correction using default Q values. In g–j, the result of GO enrichment analyses were filtered using Funset with the Benjamini-Hochberg correction (FDR threshold to 0.05) on P values determined by the hypergeometric test.