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. 2022 Jun 16;13:3462. doi: 10.1038/s41467-022-31208-4

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a Global distribution of 205 of the 207 wild C. elegans strains that were obtained from CeNDR and used in this study. Strains are colored by their sampling location continent (yellow, Europe; magenta, North America; pink, South America; light blue, Africa; dark blue, Australia; green, Asia), except for Hawaiian strains (red). The two strains missing on the map are lacking sampling locations. b Graphic illustration of the workflow to acquire C. elegans transcriptome data. Created using BioRender.com. c Estimated developmental age (y-axis) of 207 wild C. elegans strains (x-axis), of which 147 strains had three replicates and 60 strains had two replicates. Strains on the x-axis are sorted by their mean estimated age from two to three biological replicates. Error bars show the standard deviation of estimated age among replicates of each strain. d, e Two neighbor-joining trees of the 207 C. elegans strains using 598,408 biallelic segregating sites in non-divergent regions (d) and expression of 22,268 transcripts in non-divergent regions (e) are shown. Strains in c–e are colored as in a.