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. 2022 Jun 16;13:3167. doi: 10.1038/s41467-022-30828-0

Fig. 2. Direct activation mechanism of of the mGlu1 mutant by dA-CBC.

Fig. 2

a Pd(bpy)-induced mGlu1 responses in HEK293 cells expressing the mGlu1 mutant. Left: representative traces of Ca2+ responses induced by 10 µM of Pd(bpy) in HEK293 cells expressed with the mGlu1 N264H (red), P58H (gray), A59H (blue), or K61H (green) mutants. Right: averaged Δratio. (n = 20). P = 1.271 × 10–6 for N264H. (One-way ANOVA with Dunnet’s test, ***P < 0.001). b Mutated coordinating amino acid residues within 15 Å from N264 in the crystal structure of the VFT domain (PDB 1EWK). c Averaged Δratio induced by 10 µM Pd(bpy) (left, P = 2.036 × 10–6, 5.532 × 10–6 for E60A/N264H, H111F/N264H, respectively) and 10 µM glutamate (right, P = 1.349 × 10–4 for H54F/N264H). (n = 20). (One-way ANOVA with Dunnet’s test, ***P < 0.001). Data are presented as mean ± s.e.m.