Figure 1.

Aβ overload lowers the mitochondrial mass. Mitochondrial mass was studied by analysing the levels of the beta subunit of mitochondrial Complex V (CxVβ) as structural protein. The levels of CxVβ in cerebral cortex (A) and hippocampus (B) from 3-, 6- and 12-month-old wt and APP/PS1 mice are shown. Upper panels show histograms representing the protein densitometric analysis and representative experiments are presented in the bottom panels. Data are expressed as mean ± SEM; n = 5–10 mice. *p < 0.05; ***p < 0.001. Statistical significance was assessed by two-way ANOVA followed by Tukey’s post hoc test for multiple comparisons. CxVβ levels in rat primary neurons (C) and SH-SY5Y cells (D) treated with 1 µM oligomerised Aβ₄₂ for 24 h. Upper panels show the protein levels estimation and representative immunoblots are reflected at the bottom. Data are expressed as mean ± SEM; primary neurons: n = 7, SH-SY5Y cells: n = 6. *p < 0.05; ***p < 0.001. Statistical significance was assessed by student’s t-test.