Figure 5.

Overexpression of Hmgcs2 is sufficient to induce 3HBA production, antioxidative stress factors, PPARγ, and lipogenic factors in adipocytes. On day 5 after 3T3-L1-TetON-empty and 3T3-L1-TetON-Hmgcs2 adipocytes were differentiated, the adipocytes were treated with 2 µg/mL doxycycline for 48 h. On day 7 after differentiation, these adipocytes were maintained in serum-free DMEM composed of 25 mM glucose and 1 nM insulin for 24 h, followed by harvesting on day 8 after differentiation. (a) Western blot of Hmgcs2. n = 1. (b) qRT–PCR of Hmgcs2. n = 3. (c) 3HBA concentration in culture supernate. n = 3. (d–h) qRT–PCR of antioxidative stress factors. n = 3. (i) qRT–PCR of PPARγ. n = 3. (j–n) qRT–PCR of lipogenic factors. n = 3. Data are mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001. A.U., Arbitrary Unit.