Figure 7.

Hmgcs2 regulates antioxidative stress factors and ROS, PPARγ, and lipogenic factors in an autocrine manner by endogenous 3HBA production in adipocytes. On day 3 after 3T3-L1 adipocytes were differentiated, siRNA was introduced by reverse transfection for 48 h, and forward transfection was performed for 48 h. On day 7 after differentiation, the 3T3-L1 adipocytes were maintained in serum-free DMEM composed of 25 mM glucose and 1 nM insulin for 24 h, followed by harvest on day 8 after differentiation. (a) qRT–PCR of Hmgcs2. (b) 3HBA concentration in cell lysate. n = 3. (c–g) qRT–PCR of antioxidative stress factors. n = 3. (h) Cellular ROS detected by 2′,7′-dichlorofluorescein diacetate (DCFDA) assay. n = 3. (i,j) qRT–PCR of PPARγ and Adiponectin. n = 3. (k–o) qRT–PCR of lipogenic factors. n = 3. Data are mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001. A.U., Arbitrary Unit.