Figure 3:
Retentate cell-free Hb concentration (A) over 10 diacycles of RBC washing using TFF. 10 single RBC units were processed using the TFF RBC washing system. An ANOVA test was performed on the data subsets 0× to 10× diacycles, 0× to 4× diacycles, and 4× to 10× diacycles (α = 0.05, Ho = Hb concentration is independent of the diacycle). Significance was found within the 0× to 10× diacycles (p = 8E-16, ***), and 0× to 4× diacycles (p = 2.8E-7, ***) subgroups. Significance was found between the 0× diacycle and 1×, 2×, 3×, and 4× diacycles using TukeyHSD (p = 0.001, 2.9E-5, 1.6E-6, and 7E-7 respectively). No significance was found within the 4× to 10× diacycles subgroup (p = 0.458, NS). The dotted line indicates 1% hemolysis. Permeate cell-free Hb concentration (B) over 10 diacycles of RBC washing using TFF. An ANOVA test was performed on the data subsets 1× to 10× diacycles, 1× to 4× diacycles, and 4× to 10× diacycles (α = 0.05, Ho = Hb concentration is independent of the diacycle). There was significance within the data for 1× to 10× diacycles (p = 6.2E-15, ***), and for 1× to 4× diacycles (p = 1.6E-5, ***). Within the 1× to 4× diacycle subgroup, significant differences were found between the 1× diacycle and 2×, 3×, 4× diacycles using TukeyHSD (p = 0.006, 0.0001, and 3.2E-5 respectively). No significance was found within the 4× to 10× diacycle (p = 0.151, NS).