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. 2022 Jun 3;12:862806. doi: 10.3389/fonc.2022.862806

Figure 1.

Figure 1

PTEN expression and microenvironmental interactions affect the response to molecular targeted inhibition in isogenic CRC cell lines. HCT116 Parental and HCT116 PTEN-/- cell lines were treated with increased doses (dose range 0.1-1000 nM) of Geda or Geda and Tram combination (1:1) (A, B, respectively) in serum-free condition, fibroblast HFF derived CM (left panels) or direct CRC/GFP-tagged HFF co-culture (right panels). Cell viability for wo FBS and HFF-CM was assessed by Crystal Violet assay after 72 h of treatment. For HFF co-culture cells were counted after 72 h of treatment and the number of GFP positive and negative cells were calculated using cytofluorimetric analysis. Results are expressed as percentage of growth inhibition relative to untreated control and represent the average ± SEM of three independent experiments. Asterisks indicate statistically significant differences (p-value < 0.05 by 2-tailed Student’s t test) for the comparison between treatment under wo FBS and HFF-CM or co-co conditions.