FIG. 3.

Effects of solvents on copper toxicity sensing in S. cerevisiae LucΔ. The presence of 9% ethanol (■) resulted in an increase in the toxic response, and a loss in sensitivity was observed for 9% methanol (□), compared to the dose-response curve for double-distilled H₂O (▵). The presence of 9% acetone (●) resulted in a dose-response curve similar to that for double-distilled H₂O. These experiments were carried out in triplicate at 25°C, and the error bars represent standard errors of the mean triplicate value. For comparative assays the same biosensor sample was used; however, the assays were repeated on at least three separate occasions and the trends were found to be identical.