FIG. 4.
Enhanced survival of E. coli BL21DE3(pSpsA) after immobilization on nylon membranes, storage at −20°C, and rehydration with LB medium at 37°C. (a) BL21DE3(pT7-7) spotted onto untreated membrane. (b) BL21DE3(pT7-7) spotted onto treated (100 mM trehalose) membrane. (c) BL21DE3(pSpsA) spotted onto untreated membrane. (d) BL21DE3(pSpsA) spotted onto treated (100 mM trehalose) membrane.