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. 2000 Apr;66(4):1680–1684. doi: 10.1128/aem.66.4.1680-1684.2000

FIG. 4.

FIG. 4

Enhanced survival of E. coli BL21DE3(pSpsA) after immobilization on nylon membranes, storage at −20°C, and rehydration with LB medium at 37°C. (a) BL21DE3(pT7-7) spotted onto untreated membrane. (b) BL21DE3(pT7-7) spotted onto treated (100 mM trehalose) membrane. (c) BL21DE3(pSpsA) spotted onto untreated membrane. (d) BL21DE3(pSpsA) spotted onto treated (100 mM trehalose) membrane.