FIGURE 3.

Effects of combination therapy on DNA damage. Jeko-1 and Granta-519 cells were treated with ganetespib (15 nM for Jeko-1 and 45 nM for Granta-519 cells, respectively) for 12 h, and then treated with either ibrutinib at 6 μM or vehicle for 60 h. (A–D) Representative pictures and statistics of immunofluorescence of 53BP1 in Jeko-1 (A,B) and Granta-519 cells (C,D). (E–H) Representative pictures and statistics of immunofluorescence of γH2AX in Jeko-1 (E,F) and Granta-519 cells (G,H). Mean ± SD, n = 3.