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Identified conformational variations of ART WT, S428A, and P429A in cells or after isolation via FLAG-tagged immunoaffinity purification and a high salt wash.
| Cytoplasm | Nucleus | |||||
|---|---|---|---|---|---|---|
| ATR isomer | WT | S428A | P429A | WT | S428A | P429A |
| In cell | Cis/trans | Cis | Trans | Trans | Trans | Trans |
| Purified | Cis | Cis | Trans | Cis | Cis | Trans |
