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. 2022 Jun 3;10:854115. doi: 10.3389/fcell.2022.854115

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Copyright © 2022 L, JG, I, M and P.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Olfr601 KO sperms are able to fertilize eggs. (A) Litter size after mating of WT or Olfr601 KO males with WT females. Columns display mean ± s.e.m. Numbers within each column indicate the number of males tested. ANOVA p > 0.05. (B) In vivo fertilization rates after mating of WT or Olfr601 KO males with super-ovulated WT females. Columns display mean ± s.e.m. Numbers within each column indicate the number of males tested. ANOVA p > 0.05. (C) In vitro fertilization rates following co-incubation of WT or Olfr601 KO sperms and WT oocytes. Columns display mean ± s.e.m. Numbers within each column indicate the number of males tested. ANOVA p > 0.05. (D) Sperm-egg fusion assay. Sperm lacking OLFR601 fused with Hoechst pre-loaded zona-free eggs, which transferred the stain to them upon membrane fusion.