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. 2022 Jun 3;13:840567. doi: 10.3389/fphar.2022.840567

FIGURE 5.

FIGURE 5

MGF-regulated mitogen-activated protein kinase (MAPK) signaling in vivo and in vitro. (A) MGF structure. (B) Potential protein targets of MGF ranked by the standard score of the probabilities. (C) Immunoblotting analysis of p-JNK, JNK, p-p38, p38, p-ERK, ERK, and β-tubulin protein levels in the hippocampus of mice. The number represents the normalized quantitative value of the protein. (D) The schematic representation of LPS stimulation in BV2 cells. (E) Immunoblotting analysis of iNOS, p-JNK, JNK, p-p38, p38, p-ERK, ERK, and β-tubulin protein levels from BV2 cells after being treated with MGF for 0.5 h and then stimulated LPS (1 μg/ml) for 6 h. The number represents the normalized quantitative value of the protein. (F–H) RT-PCR analysis of TNF-α, IL-6, and IL-1β mRNA levels in BV2 cells after being treated with MGF for 0.5 h and then stimulated LPS (1 μg/ml) for 6 h. Error bars are mean ± SEM. *p < 0.05, **p < 0.01, and ***p < 0.001.