Figure 1. Characterization of real doublets in a mixture of three human lung adenocarcinoma cell lines.

A: Observed median (and +/- one median absolute deviation in) library sizes per cell type against additive expectation for single cell and doublet types in a real dataset. The dashed line indicates the diagonal. B: Relative contribution of composing cell types in real doublets (each point represents a doublet) plotted against the expected relative contributions (based on the ratio between the median library sizes of the composing cell types). Values indicate the relative contribution of one of the two cell types to the doublet’s transcriptome. The dashed line indicates the diagonal, and the thick line indicates the weighted mean per doublet type. The annotation of cell types and their combinations comes from the original Demuxlet analysis by Tian et al., excluding ambiguous calls.