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. 2022 Jun 3;9:904098. doi: 10.3389/fmolb.2022.904098

FIGURE 6.

FIGURE 6

Representative IHC analyses of p53/Ki-67/MGMT/IDH1R132H protein expression in cancer cells of glioblastoma patients. (A) Representative glioblastoma with HE staining. (B) Normal/wild-type p53 protein expression pattern with partly and weakly positive expression in tumor nuclei. Two patterns were identified as abnormal/mutant-staining pattern. (C) Abnormal overexpression of p53 protein with strong staining in nearly all tumor nuclei compared to internal control central fibroblasts. (D). Abnormal complete absence of p53 staining with sufficient staining of internal controls (fibroblasts, endothelial cells, or lymphocytes). (E) Low proportion of Ki-67 protein expression in tumor nuclei suggested that the tumor has low proliferative activity. (F) High proportion of Ki-67 protein expression in tumor nuclei suggested that the tumor has high proliferative activity. (G) Negative expression of MGMT protein in tumor nuclei might be related to MGMT methylation. (H) Strong positive expression of MGMT protein in tumor nuclei. (I) IDH1 R132H wild-type protein expression pattern with cytoplasmic negative staining of tumor cells. (J) IDH1 R132H mutation protein expression pattern with cytoplasmic positive staining of tumor cells. All mages were taken at 10 ×10 magnification on the Leica DM2000 microscope.