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. 2022 Jun 3;13:899609. doi: 10.3389/fimmu.2022.899609

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Copyright © 2022 Cannon, Holloman, Wilson, Miranda, Dopkins, Nagarkatti and Nagarkatti

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Analysis of gene dysregulation in Neutrophils. C57/BL6 mice were injected with ConA followed by vehicle or TCDD 1 hour later as described in Figure 1 legend. Liver infiltrating cells were isolated and scRNA-seq was performed as described in Methods. Heatmaps consisting of the top 50 differential genes per treatment were generated using Loupe Browser. Heatmaps of differentially expressed genes from (A) Neutrophils. In panel (A), the X marked with some genes represents an expression level over 20. (B–E) Violin plot of Log2 expression of target genes.