Figure 7.

Fasciola hepatica FABP1 modulates T cell polarization in vivo. Mice were injected sc into the hind hock with PBS (black bars) or 25 µg recombinant fhFABP1 (orange bars) together with or without LPS (200 ng) The draining popliteal lymph nodes (LNs) were collected after 6 days for FACS analyses and ex vivo restimulation of LN cells (A) The total LN cells count (B) and the frequencies of CD4⁺ T cells (C) and CD4⁺CD44^high effector T cells (D) were determined The percentages of IL-4⁺, INFγ⁺ and IL-10⁺ cells within the CD4+CD44^high effector T cell population were also assessed, and the IL-4-on-IFNγ ratio plotted (E) Alternatively, part of the LN cells were re-stimulated ex vivo and secretion of IL-4 and IFNγ were measured by cytokine bead assay (F). Results are expressed as mean ± SEM * P≤ 005 vs LPS alone, ^#P ≤ 005 vs PBS (n=3-5 mice per group).