FIGURE 2.

Genetic approaches toward achieving cell-type specificity. (A) Schematic of labeling neurons with different reporters using Cre (red) and Flp (green) recombinases separately. Cre and Flp-positive cells are visualized in yellow. (B) Schematic of labeling neurons with both Cre and Flp recombinases using the Con-Fon reporter, which is designed to be expressed only when both Cre and Flp recombinases exist. (C) Schematic of TRAP. The reporter of interest will be expressed only when Tamoxifen (TM) and neural activity (as indicated by cfos expression) exist in a TRAP + neuron. (D) Schematic of FLiCRE. When light and neuronal activity (as measured by an increase in calcium concentration) exist simultaneously, neurons will express the FLiCRE reporter of interest.