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Retrograde viral tracing strategies. (A) Non-transsynaptic retrograde tracing using retro-AAV or CAV-2. (B) Transsynaptic retrograde tracing using pseudotyped rabies virus is used to trace monosynaptic connections. Rabies G (RB) is provided in trans and neurons are pseudotyped with TVA receptor to endow cell-type specificity. Once a neuron expressing TVA and RG is infected with RABV expressing EnvA, the RABV may jump across one synapse in a retrograde manner.
