Figure 2.

Iboxamycin is bacteriostatic against L. monocytogenes. Exponentially growing L. monocytogenes type strains; 10403S (a and d), EDG-e (b and e) or VgaA-deficient EDG-e Δlmo0919 (c and e) were treated with 4 × MIC of either iboxamycin, clindamycin or lincomycin or no antibiotic as control. Cells were harvested at given timepoints and washed before plating. After 24 (a–c) or 48 h (d–f) of incubation, colonies were counted to determine cfu/mL. All experiments were carried out in MH-F broth at 37°C without shaking, data points are from three biological replicates and standard deviation is indicated with error bars.